Rat-urine glycosidases and kidney damage

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Rat-urine glycosidases and kidney damage.

1. The activities of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl, beta-glucosaminidase were estimated in normal and pathological rat urine, with 4-methylumbelliferyl glycosides as substrates. 2. Kidney damage induced by injections of uranium nitrate, mercuric chloride, potassium dichromate or 4-nitrophenylarsonic acid causes a marked increase in the urinary excretion o...

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Diagnostic potential of serum and urine glycosidases in acquired diseases

Glycosidases are generally glycoproteins from lysosomal origin that catalyze the hydrolysis of glycoproteins, glycolipids and glycosaminoglycans, as well as synthetic substrates. They accomplish a very important role (partially known) in physiological circumstances, and their dysfunction causes pathological processes, where they have been mainly studied in connection to the lysosomal storage di...

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Hydrogen sulfide ameliorates the kidney dysfunction and damage in cisplatin-induced nephrotoxicity in rat

Hydrogen Sulfide (H2S) prevents and treats a variety of disorders via its cytoprotective effects. However, the effects of H2S on rats with cisplatin (CP) nephrotoxicity are unclear. The aim was to study the effects of H2S on rats with CP nephrotoxicity. Thirty male Sprague-Dawley rats were divided into three groups: control group, nephrotoxic group received sing...

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Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.    Materials and Methods: IR...

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Purification and immunological quantification of rat liver lysosomal glycosidases.

Although lysosomal enzyme activities are known to vary in response to numerous physiological and pharmacological stimuli, the relationship between lysosomal enzyme activity and enzyme concentration has not been systematically studied. Therefore we developed radioimmunoassays for two lysosomal glycosidases in order to determine lysosomal enzyme concentration. beta-Galactosidase and beta-glucuron...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1967

ISSN: 0006-2936

DOI: 10.1042/bj1020533